Immune safety of a novel oncolytic mutant M1 after administration in vivo / 华中科技大学学报（医学）（英德文版）

The aim of this study was to evaluate the safety and efficiency of a novel, oncolytic adenovirus mutant M1 administered in conjunction with immunosuppressive agents. Animal models were established by administering purified M1 either intravenously or retroperitoneally. At different time points, blood samples were taken from the mice for testing of liver and renal function. Microscopic examination of the liver was performed to observe pathological changes. Immunohistochemical analyses were used to evaluate the expression of the adenovirus in the liver. Lymphocyte recruitment to the liver and the activation of adenovirus specific T cells were also analyzed. No signs of general toxicity were observed, but transient increases in ALT and Scr were observed following the administration of M1. Microscopic examination revealed a mild inflammatory response in the liver. Compared to intravenous injection, higher expression levels of adenoviral proteins were observed after retroperitoneal injection. Combined treatment with cyclosporine A resolved the liver and kidney dysfunction and increased the concentration of the adenovirus in the liver. The use of the novel oncolytic adenovirus mutant M1 in vivo is safe, and the combined administration of M1 with immunosuppressive agents was able to enhance the effectiveness and safety profile of M1.

Immune safety of a novel oncolytic mutant M1 after administration In Vivo / 华中科技大学学报（医学）（英德文版）

The aim of this study was to evaluate the safety and efficiency of a novel, oncolytic adenovirus mutant M1 administered in conjunction with immunosuppressive agents. Animal models were established by administering purified M1 either intravenously or retroperitoneally. At different time points, blood samples were taken from the mice for testing of liver and renal function. Microscopic examination of the liver was performed to observe pathological changes. Immunohistochemical analyses were used to evaluate the expression of the adenovirus in the liver. Lymphocyte recruitment to the liver and the activation of adenovirus specific T cells were also analyzed. No signs of general toxicity were observed, but transient increases in ALT and Scr were observed following the administration of M1. Microscopic examination revealed a mild inflammatory response in the liver. Compared to intravenous injection, higher expression levels of adenoviral proteins were observed after retroperitoneal injection. Combined treatment with cyclosporine A resolved the liver and kidney dysfunction and increased the concentration of the adenovirus in the liver. The use of the novel oncolytic adenovirus mutant M1 in vivo is safe, and the combined administration of M1 with immunosuppressive agents was able to enhance the effectiveness and safety profile of M1.

Advance of researches on thyroid tissues autotransplantation and embryonic stem cell transplantation in therapy of hypothyroidism / 生物医学工程学杂志

Patients with irreversible hypothyroidism require lifelong levo-thyroxin ( L-T4) replacement therapy, which makes them feel discomfortable. With the development of the thyroid tissues autotransplantation and embryonic stem cell (ESC), this would be a more physiological approach to the treatment of irreversible hypothyroidism. The animal experiments and human clinical trials on thyroid tissues autotransplantation have shown that the autograft can survive and function. The advanced researches have demonstrated that ESC can differentiate into thyroid follicular cells.

Diagnosis and treatment of recurrent urethral stricture with false passage / 中国微创外科杂志

Objective To evaluate diagnostic methods and long-term curative effects for recurrent urethral stricture with false passage. Methods Among a total of 620 cases of urethral stricture or atresia treated from March 1987 to March 2005 in this hospital,false passage was present in 40 cases(6.4%).Diagnostic methods included urethrography,sonourethrography,injection of methylene blue into the bladder by cystostomy,CT and/or MRI examinations,or exploration by vesicotomy.Surgical methods included insertion of a memory metallic stent after urethrotomy in 10 cases,electrotomy in 7 cases,urethral reunion in 4 cases,hydroelectric shock wave therapy in 8,posterior pull-through urethroplasty in 4,patient self-administered urethral dilation in 5,excision of false passage in 1,and perineourethrostomy in 1.Results All the 40 cases were followed for 1~15 years(mean,9 years).The urethral stricture was cured in 32 cases(80.0%),and therapeutic failure was observed in 8 cases(20.0%),including 4 cases of unstable pelvic fracture.Conclusions Sonourethrography is the most accurate noninvasive method of staging urethra strictures.It is simple to perform,requires no radiation,and offers a dynamic three dimensional measurement.

Treatment of prostatic pelvic floor muscular spasm syndrome by transurethral low-frequency pulse electrotherapy / 中国微创外科杂志

Objective To explore the clinical effects of low-frequency (50Hz) pulse electrotherapy (LFPE) in the treatment of prostatic pelvic floor muscular spasm syndrome (PPMS), and the influence of this procedure on the temperature of rabbit urethral mucosa. Methods (1) A series of 60 cases of PPMS had undergone LFPE; (2) Thirty-six rabbits were randomly divided into 6 groups: 3 experimental groups were treated for 15 min, 30min and 50 min, respectively, while the other 3 groups served as control groups. Results All cases had been followed for (1～16) months, with a mean of 6 months. Of the 60 cases, 12 were cured, 38 improved, and 10 failed,effective rate being 83.3%(50/60). In animal experiment, there were no significant differences between the experimental and control groups on temperatures of rabbit urethral local mucosa (t=1.3306, P=0.1049). Conclusions The study demonstrates that 50Hz pulse electrotherapy may be effectively applied to PPMS.

INVESTIGATION OF THE INFLUENCE MENTAL BEHAVIOURS OF SOLDIERS DURING PERFORMING A TASK OF EMERGENCY / 解放军医学杂志

To probe influence of stress on the mental behaviours of soldiers. ①The mental behaviours during performing a task of emergency were investigated in 1033 soldieries. Of them, 200 performed the task of cracking smuggling activities, 338 the tasks of fighting a flood and sending relief to a disaster area, 187 the task of safeguarding the public order, and 98 task of pursuing and capturing escaped criminals, 210 the task of being on duty of internal conference of heads of state, and 200 as controls. ②The adult male rats were divited in to 4 groups and different stress impairment models were established. Spatial distinction ability was measured with jump platform test, avoidance action ability was determined with avoid lightless test. The results showed that the most servere stress was found in those who persued and captured the escaped criminals. Insomnia, amnesia,ballucination and loss of confidence were found in the remainders. It is suggestted that stress can lead to decrease in memory and response of human beings and rats.